abstract：:Moorella thermoacetica can grow with H₂ and CO₂, forming acetic acid from 2 CO₂ via the Wood-Ljungdahl pathway. All enzymes involved in this pathway have been characterized to date, except for methylenetetrahydrofolate reductase (MetF). We report here that the M. thermoacetica gene that putatively encodes this enzyme,...

journal_title：Journal of bacteriology

authors： Mock J,Wang S,Huang H,Kahnt J,Thauer RK

更新日期：2014-09-01 00:00:00

abstract：:The crystal structure of the Na(+)-coupled melibiose permease of Salmonella enterica serovar Typhimurium (MelBSt) demonstrates that MelB is a member of the major facilitator superfamily of transporters. Arg residues at positions 295, 141, and 363 are involved in interdomain interactions at the cytoplasmic side by gove...

journal_title：Journal of bacteriology

authors： Amin A,Ethayathulla AS,Guan L

更新日期：2014-09-01 00:00:00

abstract：:The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of Brucella biology is...

journal_title：Journal of bacteriology

authors： Zúñiga-Ripa A,Barbier T,Conde-Álvarez R,Martínez-Gómez E,Palacios-Chaves L,Gil-Ramírez Y,Grilló MJ,Letesson JJ,Iriarte M,Moriyón I

更新日期：2014-08-15 00:00:00

abstract：:Chlamydiae are widespread Gram-negative pathogens of humans and animals. Salicylidene acylhydrazides, developed as inhibitors of type III secretion system (T3SS) in Yersinia spp., have an inhibitory effect on chlamydial infection. However, these inhibitors also have the capacity to chelate iron, and it is possible tha...

journal_title：Journal of bacteriology

authors： Bao X,Gylfe A,Sturdevant GL,Gong Z,Xu S,Caldwell HD,Elofsson M,Fan H

更新日期：2014-08-15 00:00:00

abstract：:The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...

journal_title：Journal of bacteriology

authors： Ishihama A,Kori A,Koshio E,Yamada K,Maeda H,Shimada T,Makinoshima H,Iwata A,Fujita N

更新日期：2014-08-01 00:00:00

abstract：:Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell...

journal_title：Journal of bacteriology

authors： Samhita L,Nanjundiah V,Varshney U

更新日期：2014-07-01 00:00:00

abstract：:The quorum-sensing (QS) system present in the emerging nosocomial pathogen Stenotrophomonas maltophilia is based on the signaling molecule diffusible signal factor (DSF). Production and detection of DSF are governed by the rpf cluster, which encodes the synthase RpfF and the sensor RpfC, among other components. Despit...

journal_title：Journal of bacteriology

authors： Huedo P,Yero D,Martínez-Servat S,Estibariz I,Planell R,Martínez P,Ruyra A,Roher N,Roca I,Vila J,Daura X,Gibert I

更新日期：2014-07-01 00:00:00

abstract：:The EcfG-type sigma factor RpoE2 is the regulator of the general stress response in Sinorhizobium meliloti. RpoE2 activity is negatively regulated by two NepR-type anti-sigma factors (RsiA1/A2), themselves under the control of two anti-anti-sigma factors (RsiB1/B2) belonging to the PhyR family of response regulators. ...

journal_title：Journal of bacteriology

authors： Sauviac L,Bruand C

更新日期：2014-07-01 00:00:00

abstract：:Human-pathogenic Bartonella henselae causes cat scratch disease and vasculoproliferative disorders. An important pathogenicity factor of B. henselae is the trimeric autotransporter adhesin (TAA) Bartonella adhesin A (BadA), which is modularly constructed, consisting of a head, a long and repetitive neck-stalk module, ...

journal_title：Journal of bacteriology

authors： Schmidgen T,Kaiser PO,Ballhorn W,Franz B,Göttig S,Linke D,Kempf VA

更新日期：2014-06-01 00:00:00

abstract：:t is the holin gene for coliphage T4, encoding a 218-amino-acid (aa) protein essential for the inner membrane hole formation that initiates lysis and terminates the phage infection cycle. T is predicted to be an integral membrane protein that adopts an N(in)-C(out) topology with a single transmembrane domain (TMD). Th...

journal_title：Journal of bacteriology

authors： Moussa SH,Lawler JL,Young R

更新日期：2014-06-01 00:00:00

abstract：:When carbon sources become limiting for growth, bacteria must choose which of the remaining nutrients should be used first. We have identified a nutrient-sensing signaling network in Escherichia coli that is activated at the transition to stationary phase. The network is composed of the two histidine kinase/response r...

journal_title：Journal of bacteriology

authors： Behr S,Fried L,Jung K

更新日期：2014-06-01 00:00:00

abstract：:We studied the activity of a debranching enzyme (TreX) from Sulfolobus solfataricus on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc₄-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation t...

journal_title：Journal of bacteriology

authors： Nguyen DH,Park JT,Shim JH,Tran PL,Oktavina EF,Nguyen TL,Lee SJ,Park CS,Li D,Park SH,Stapleton D,Lee JS,Park KH

更新日期：2014-06-01 00:00:00

abstract：:Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of t...

journal_title：Journal of bacteriology

authors： Mokrousov I,Vyazovaya A,Narvskaya O

更新日期：2014-05-01 00:00:00

abstract：:Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...

journal_title：Journal of bacteriology

authors： Tulum I,Yabe M,Uenoyama A,Miyata M

更新日期：2014-05-01 00:00:00

abstract：:Bacterial lipases play important roles in bacterial metabolism and environmental response. Our laboratory recently discovered that a novel lipoprotein lysophospholipase, VolA, localizes on the surface of the Gram-negative aquatic pathogen Vibrio cholerae. VolA functions to cleave exogenous lysophosphatidylcholine, fre...

journal_title：Journal of bacteriology

authors： Pride AC,Guan Z,Trent MS

更新日期：2014-04-01 00:00:00

abstract：:Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends on the AHL synthase SinI and two transcriptional regulators, SinR and ExpR. Our study demonstrates that ectopic expression of the gene rne, coding ...

journal_title：Journal of bacteriology

authors： Baumgardt K,Charoenpanich P,McIntosh M,Schikora A,Stein E,Thalmann S,Kogel KH,Klug G,Becker A,Evguenieva-Hackenberg E

更新日期：2014-04-01 00:00:00

abstract：:Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporul...

journal_title：Journal of bacteriology

authors： Geng Y,de Keyzer J,Scheffers DJ,Driessen AJ

更新日期：2014-04-01 00:00:00

abstract：:Acetate kinase (ACK) converts acetyl phosphate to acetate along with the generation of ATP in the pathway for mixed-acid fermentation in Lactococcus lactis. The reverse reaction yields acetyl phosphate for assimilation purposes. Remarkably, L. lactis has two ACK isozymes, and the corresponding genes are present in an ...

journal_title：Journal of bacteriology

authors： Puri P,Goel A,Bochynska A,Poolman B

更新日期：2014-04-01 00:00:00

abstract：:The sets of compounds that can support growth of an organism are defined by the presence of transporters and metabolic pathways that convert nutrient sources into cellular components and energy for growth. A collection of known nutrient sources can therefore serve both as an impetus for investigating new metabolic pat...

journal_title：Journal of bacteriology

authors： Mackie A,Paley S,Keseler IM,Shearer A,Paulsen IT,Karp PD

更新日期：2014-03-01 00:00:00

abstract：:Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI), which represent the most frequent nosocomial infections. Knowledge of genetic factors for catheter colonization is limited, since their role has not been assessed using physicochemical condit...

journal_title：Journal of bacteriology

authors： Reisner A,Maierl M,Jörger M,Krause R,Berger D,Haid A,Tesic D,Zechner EL

更新日期：2014-03-01 00:00:00

abstract：:Mevalonate (MVA) metabolism provides the isoprenoids used in archaeal lipid biosynthesis. In synthesis of isopentenyl diphosphate, the classical MVA pathway involves decarboxylation of mevalonate diphosphate, while an alternate pathway has been proposed to involve decarboxylation of mevalonate monophosphate. To identi...

journal_title：Journal of bacteriology

authors： Vannice JC,Skaff DA,Keightley A,Addo JK,Wyckoff GJ,Miziorko HM

更新日期：2014-03-01 00:00:00

abstract：:The stationary phase/general stress response sigma factor RpoS (σ(S)) is necessary for adaptation and restoration of homeostasis in stationary phase. As a physiological consequence, its levels are tightly regulated at least at two levels. Multiple small regulatory RNA molecules modulate its translation, in a manner th...

journal_title：Journal of bacteriology

authors： Thompson KM,Gottesman S

更新日期：2014-02-01 00:00:00

abstract：:Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

journal_title：Journal of bacteriology

authors： Milunovic B,diCenzo GC,Morton RA,Finan TM

更新日期：2014-02-01 00:00:00

abstract：:The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...

journal_title：Journal of bacteriology

authors： Ge X,Lyu ZX,Liu Y,Wang R,Zhao XS,Fu X,Chang Z

更新日期：2014-02-01 00:00:00

abstract：:The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...

journal_title：Journal of bacteriology

authors： Guo Q,Ahn SJ,Kaspar J,Zhou X,Burne RA

更新日期：2014-01-01 00:00:00

abstract：:We recently demonstrated that Pseudomonas aeruginosa PAO1 undergoes a pronounced phenotypic change when introduced into the intestines of rats during surgical injury. Recovered strains displayed a specific phenotype (termed the P2 phenotype) characterized by altered pyocyanin production, high collagenase activity, hig...

journal_title：Journal of bacteriology

authors： Luong PM,Shogan BD,Zaborin A,Belogortseva N,Shrout JD,Zaborina O,Alverdy JC

更新日期：2014-01-01 00:00:00

abstract：:In bacteria, a subset of membrane proteins insert into the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the translocon via a lateral gate i...

journal_title：Journal of bacteriology

authors： Li Z,Boyd D,Reindl M,Goldberg MB

更新日期：2014-01-01 00:00:00

abstract：:Peptide deformylase (PDF) is an essential bacterial metalloprotease involved in deformylation of N-formyl group from nascent polypeptide chains during protein synthesis. Iron-containing variants of this enzyme from Salmonella enterica serovar Typhimurium (sPDF) and Mycobacterium tuberculosis (mPDF), although inhibited...

journal_title：Journal of bacteriology

authors： Kumar S,Kanudia P,Karthikeyan S,Chakraborti PK

更新日期：2014-01-01 00:00:00

abstract：:Listeria monocytogenes is a Gram-positive human intracellular pathogen that infects diverse mammalian cells. Upon invasion, L. monocytogenes secretes multiple virulence factors that target host cellular processes and promote infection. It has been presumed, but was not empirically established, that the Sec translocati...

journal_title：Journal of bacteriology

authors： Burg-Golani T,Pozniak Y,Rabinovich L,Sigal N,Nir Paz R,Herskovits AA

更新日期：2013-12-01 00:00:00

abstract：:Type 3 fimbriae are adhesive organelles found in enterobacterial pathogens. The fimbriae promote biofilm formation on biotic and abiotic surfaces; however, the exact identity of the receptor for the type 3 fimbriae adhesin, MrkD, remains elusive. We analyzed naturally occurring structural and functional variabilities ...

journal_title：Journal of bacteriology

authors： Stahlhut SG,Chattopadhyay S,Kisiela DI,Hvidtfeldt K,Clegg S,Struve C,Sokurenko EV,Krogfelt KA

更新日期：2013-12-01 00:00:00

abstract：:SbmA is an inner membrane protein of Gram-negative bacteria that is involved in the internalization of glycopeptides and prokaryotic and eukaryotic antimicrobial peptides, as well as of peptide nucleic acid (PNA) oligomers. The SbmA homolog BacA is required for the development of Sinorhizobium meliloti bacteroids with...

journal_title：Journal of bacteriology

authors： Runti G,Lopez Ruiz Mdel C,Stoilova T,Hussain R,Jennions M,Choudhury HG,Benincasa M,Gennaro R,Beis K,Scocchi M

更新日期：2013-12-01 00:00:00

abstract：:Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discerned through evolutionar...

journal_title：Journal of bacteriology

authors： Singh AP,Lai SC,Nandi T,Chua HH,Ooi WF,Ong C,Boyce JD,Adler B,Devenish RJ,Tan P

更新日期：2013-12-01 00:00:00

abstract：:Ethanolamine (EA) metabolism is a trait associated with enteric pathogens, including enterohemorrhagic Escherichia coli O157:H7 (EHEC). EHEC causes severe bloody diarrhea and hemolytic uremic syndrome. EHEC encodes the ethanolamine utilization (eut) operon that allows EHEC to metabolize EA and gain a competitive advan...

journal_title：Journal of bacteriology

authors： Luzader DH,Clark DE,Gonyar LA,Kendall MM

更新日期：2013-11-01 00:00:00

abstract：:In bacteria like Escherichia coli, the accumulation of glucose-6-phosphate (G6P) or its analogs such as α-methyl glucoside-6-phosphate (αMG6P) results in stress that appears in the form of growth inhibition. The small RNA SgrS is an essential part of the response that helps E. coli combat glucose-phosphate stress; the...

journal_title：Journal of bacteriology

authors： Richards GR,Patel MV,Lloyd CR,Vanderpool CK

更新日期：2013-11-01 00:00:00

abstract：:The genome of the facultative ribulose monophosphate (RuMP) cycle methylotroph Bacillus methanolicus encodes two bisphosphatases (GlpX), one on the chromosome (GlpX(C)) and one on plasmid pBM19 (GlpX(P)), which is required for methylotrophy. Both enzymes were purified from recombinant Escherichia coli and were shown t...

journal_title：Journal of bacteriology

authors： Stolzenberger J,Lindner SN,Persicke M,Brautaset T,Wendisch VF

更新日期：2013-11-01 00:00:00

abstract：:Flavin-based electron bifurcation has recently been characterized as an essential energy conservation mechanism that is utilized by hydrogenotrophic methanogenic Archaea to generate low-potential electrons in an ATP-independent manner. Electron bifurcation likely takes place at the flavin associated with the α subunit...

journal_title：Journal of bacteriology

authors： Costa KC,Lie TJ,Xia Q,Leigh JA

更新日期：2013-11-01 00:00:00

abstract：:The c subunit of Streptococcus mutans ATP synthase (FoF1) is functionally exchangeable with that of Escherichia coli, since E. coli with a hybrid FoF1 is able to grow on minimum succinate medium through oxidative phosphorylation. E. coli F1 bound to the hybrid Fo with the S. mutans c subunit showed N,N'-dicyclohexylca...

journal_title：Journal of bacteriology

authors： Araki M,Hoshi K,Fujiwara M,Sasaki Y,Yonezawa H,Senpuku H,Iwamoto-Kihara A,Maeda M

更新日期：2013-11-01 00:00:00

abstract：:Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highl...

journal_title：Journal of bacteriology

authors： Kan S,Fornelos N,Schuch R,Fischetti VA

更新日期：2013-10-01 00:00:00

abstract：:All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...

journal_title：Journal of bacteriology

authors： Ligon LS,Rigel NW,Romanchuk A,Jones CD,Braunstein M

更新日期：2013-10-01 00:00:00

abstract：:The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...

journal_title：Journal of bacteriology

authors： Mukerjea R,Ghosh P

更新日期：2013-10-01 00:00:00